Binary solvent manager g1405 d14upb008a 186015001 sample manager g1404 g14upa762m 186015006 column heater g1403 d14uph934g 186015010 pda detector g53 mupd742a 186015032 per seller, the system is in very good condition. Typical uplc and hplc chromatogram was depicted in fig. Calculates conditions for transfer of an isocratic or gradient method from one hplc column to another. Waters has become a landmark for quality hplc columns.
The calculator allows the user to input the current hplc conditions, including column length, mobile phase composition, and flow rate. Uplc technology, which has been adopted successfully in laboratories around the world for the most demanding separations, is a highly robust, dependable, and reproducible system. Installing uhplc columns onto waters acquity uplc systems. Jan 26, 2017 this video will provide an overview of how to connect waters fittings to uplc and uhplc columns. In 2005, beh technology was expanded to analytical and preparative particle sizes 2. Please note all calculations are approximations only. Aug 09, 2017 did you know that phenomenex lc columns are a universal fit for all hplc and uhplc systems on the market. Acquity uplc mclass columns are designed specifically for the waters acquity uplc mclass system and used in nano to microscale lc separations. Before starting any operation check that the high pressure limit is set to 1400 psi. When you develop methods, follow the protocol for the adoption of analytical. Consult your it department to see if you have permission to download this file. The acquity uplc system is designed to exploit the performance and efficiency of sub2 m column technology, without compromising your ability to run hplcscale separations with ease. Waters acquity uplc hclass series system manual pdf. Pharmaceutical chromatography waters corp acquity uplc.
This calculator is designed for general comparison of 2. Additional information on using the acquity uplc columns calculator can be found in a published application note. With over 40 years as a leader in lc column technology, you can rely on the quality of thermo scientific hplc and uhplc columns. Preparative scaling calculator phenomenex uhplc, hplc, spe. The high resolving power of these columns is due in part to fullyporous particles with an optimal concentration of amide ligands for associated applications. As vendors entered the market with similar instruments uhplc was coined as a way to refer to instruments similar to. The hplc columns are destroyed if the pressure overrides 2000 psi. Acquity uplc columns calculator runs on the following operating systems. Several factors influence loading capacity of columns. It is calculated according to the following equation.
Different types of hplc columns are used in analysis of different pharmaceutical compounds according to their nature and column separation capacity. So take off you lab coat, relax and let our web tool do the work. Analytical to prep gradient calculator, tubing backpressure and splitter calculator, uplc to focused gradient calculator, column backpressure and mass loading calculator. Being the most common principle hplcuhplc separation. Waters acquity uplc columns calculator the console calculator will geometrically scale an original hplc method to a new uplc method. Combine this with several column management options for both uplc and hplc, and a range of column chemistries, and youll. Uhplc calculator fortis technologies hplc columns for. How can i scale a gradient method from a uplc column to a. How can i scale a gradient method from a uplc column. Preparative scaling calculator phenomenex uhplc, hplc.
Accommodates up to four columns, in parallel column size. Download the waters acquity uplc system pdf brochure. Page 44 using the waters acquity uplc columns calculator ensures the best results for transferring the lc method from hplc to uplc or uplc to hplc. How to connect hplc columns to waters lc systems youtube. Waters acquity uplc with photodiode array pda detector compound identification with spectral confidence. Refer to the attached release notes for additional information and restrictions. It was initially added to our database on 05082008. Short columns great for fast analysis and useable on non uhplc instruments. In order to translate a method from analytical to uhplc and to find out how much time and solvent will be saved we have produced a handy calculator to aid you. Video to show how to put waters fittings and connections for hplc columns onto waters lc systems. Whether you need a highend 1500 bar system or are looking for a simpletouse nanolc, learn how the innovative and proven thermo scientific vanquish hplc and uhplc systems, the established ultimate 3000 systems, and the sensitive nano, capillary, and microflow rate systems can help you separate your science from the status quo.
We have 1 waters acquity uplc hclass series manual available for free pdf download. Significant advances in instrumentation and column design enabl. Columns are the main component in hplc because the column is responsible for the separation of the sample components. Both hplc and uplc methods can be run on the acquity uplc hclass system software acquity columns calculator for proper transfers streamline verification and validation testing with empower method validation manager. Utilizing xbridge hplc columns for method development at ph. Hplc and uhplc columns thermo fisher scientific in. Our wide range of phases offers excellent alternatives to acquity, atlantis, deltapak, novapak, resolve, spherisorb, symmetry, sunfire, xbridge, xselect. Comparison of uplc and hplc for analysis of dietary folates. Acquity uplc hss pfp is a nonendcapped silicabased column designed for low ph separations that require alternative selectivity compared to a fully endcapped, high coverage c18 phase.
Beh c 18, hss beh c 18, hss t3 and beh shield rp c 18 all the columns were designed for uplc from waters corp. The sample passes through the column with the mobile phase and separates in its. Waters acquity uplc with photodiode array pda detector. Development and validation of uplc method for quality.
How to connect waters fittings to uplc and uhplc columns duration. Did you know that phenomenex lc columns are a universal fit for all hplc and uhplc systems on the market. This now provides the method developer to fully utilize ph as a selectivity tool. Its simple to install phenomenex uhplc columns onto waters acquity uplc systems. Hplc columns hplc column selectivity measurements of more. Rarely used, regular maintenance, columns and tubes. These columns are optimized for maximum sample recovery and deliver superior chromatographic resolution through efficient use of sub2.
Acquity uplc glycan beh columns utilize hybridsilica beh technology particles functionalized with a stable, amidecontaining species. Higher flow rates and increased throughput result in superior resolution and sensitivity. Acquity uplc columns calculator is a shareware software in the category miscellaneous developed by acquity uplc columns calculator. Waters acquity uplc hclass series system manual pdf download. Free convertisseur pour colonnes acquity uplc download software at updatestar 1,746,000 recognized programs 5,228,000 known versions software news. Allows method scaling from microbore through preparative column range. The f s factor describes the similarity of two columns. A small f s indicates that two columns are very similar. This document is an addendum to the acquity uplc system operators guide.
Hplc columns hplc column selectivity measurements of. This video will provide an overview of how to connect waters fittings to uplc and uhplc columns. Download the waters acquity uplc system accessories pdf brochure. Jan 25, 2017 video to show how to put waters fittings and connections for hplc columns onto waters lc systems. H 1 h 2 is the difference between the hydrophobicity parameters of columns 1 and 2 s 1 s 2 is the. Hplc and uhplc systems thermo fisher scientific br. Use an online columns calculator see additional information to download to determine the estimated back pressure based on your variables.
Free convertisseur pour colonnes acquity uplc download. The waters acquity uplc columns calculator provided with your acquity uplc software and also available on waters web site automates the scaling calculations required to convert isocratic or gradient hplc methods to uplc methods. Waters columns are characterized by their high batchtobatch reproducability, since waters is manufacturating both, filling materials and column housings themselves. Make your method development column choices easier to visualize in an interactive manner. Consult your it department to see if you have permission to download.
In addition, method development and method validation kits are available to ensure. What size and type of column is used in the waters supported total solution accqtag ultra uplc kits for amino acids. The scaled gradient method is based on estimates of column volumes and. Get detailed information, downloads, screenshots, latest updates, news, and special offers for acquity uplc columns calculator software at updatestar the social software search engine. When developing the uplc method, four different rp c 18 columns. The waters acquity uplc system can not only dramatically increase your analytical resolution, sensitivity, and speed see how it can reduce your labs environmental footprint. Columns calculator for a target system, with a smaller volume, use an isocratic hold to account for the dwell volume differences. These new kits contain both uplc and hplc columns, with specified chemistry, and an acquity uplc columns calculator, all designed to take the guess work out of method transfer.
A large f s factor indicates that two columns are very different. As vendors entered the market with similar instruments uhplc was coined as a way to refer to instruments similar to uplc since uplc is a waters trademark. At the heart of the system was waters second generation hybrid, the ethylene bridged hybrid beh particle. In 2004, waters launched the technique known as ultraperformance lc uplc based upon sub 2micron porous particles and trademarked the acronym uplc. Additionally, you can install the columns calculator software on any personal computer with windows 7 32bit or windows 7 64bit operating systems, while logged in as a local administrator. The latest version of acquity uplc columns calculator is currently unknown. View online or download waters acquity uplc hclass series system manual. The highest pressure applied for routine analysis is 1400 psi. Search and browse videos enter terms to search videos. An overview of the columns calculator to assist you in converting hplc methods to uplc methods. The scaled gradient method is based on estimates of column volumes and is therefore only an approximation. In this study, uplc method and hplc method were established for simultaneous quantification of three curcuminoids. Running hplc methods on an acquity uplc system mafiadoc. We stock more than 5,000 hplc columns by waters in different configurations.
Pharmaceutical chromatography waters corp acquity uplc h. Columns and consumables acquity uplc columns by waters. Waters acquity uplc hclass series pdf user manuals. Acquity uplc columns calculator shareware more info. In fact, compared with traditional hplc, uplc uses columns packed with solid phase particles smaller than 2 mm, which yields a lower plate height and allows overall separation with better. Xbridge c18 columns are stable in phosphate buffer ph 12 mobile phase conditions, allowing for full use phosphate buffer across the ph 212 range. Acquity uplc columns calculator for the transfer of the usp assay monograph for levonorgestrel and ethinyl estradiol tablets.
H 1 h 2 is the difference between the hydrophobicity parameters of columns 1 and 2 s 1 s 2 is the difference between the steric parameters of columns 1 and 2 a 1 a 2 is the difference between the hydrogen bond acidity parameters of columns 1 and 2 b 1 b 2 is the difference between the hydrogen bond basicity parameters of columns 1. Wkb29941 what is the part number for the end connector kit to use 3. Work at higher efficiencies with a much wider range of linear velocities, flow rates, and backpressures. What is the best step to transfer hplc method to uplc method. Gives you the ability to select methods development column kits, choose columns by vendor or by ligand type and quickly find equivalent column chemistries for existing methods, saving you time in your laboratory. How to connect waters fittings to uplc and uhplc columns. Utilizing xbridge hplc columns for method development at. Program fileswaters acquity uplc columns calculator columnselector. Managing method transfer in the pharmaceutical laboratory. Quickly and easily reversed hplc column develop methods based on compound type, usp methods, application, or desired column phase. For nonacquity uplc systems, or if you cannot locate the calculator on your acquity uplc system. In addition, method development and method validation kits are available to ensure efficient method development and robustness.